Characterization of Verotoxigenic Escherichia Coli Isolated from Slaughtered Small Ruminants and Sla
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- Category: Biological Sciences
- Published: Sunday, 06 May 2012 03:00
- Written by Nafe' M. AL- Tawara
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Abstract
Characterization of verotoxigenic Escherichia coli isolated from slaughtered small ruminants and slaughterhouses in southern Jordan
Nafe' M. AL- Tawara
Mu'tah University, 2007
The emergence of Verotoxin-producing Escherichia coli (VTEC) as zoonotic food-borne pathogens has become a public health concern because of its life threatening human diseases. Rapid identification is important for meat hygiene management and prompts epidemiological investigations. This study was conducted to determine the prevalence rate of VTEC in slaughtered sheep and goats and to evaluate the contamination rate of VTEC in slaughterhouses and butcher shops in southern Jordan. Among collected animals' fecal samples (n =402) and slaughterhouses/butcher shops samples (n = 57) about 201 and 33, respectively, E. coli isolates were characterized by multiplex PCR reaction for detection of stx1, stx2, eaeA and E-hly A virulent genes. Throughout the study, twenty six virulent E. coli isolates had been characterized by mPCR to 7 different virulent patterns, stx1, stx1+stx2, stx1+eaeA, stx1+E-hlyA, stx1+eaeA+E-hlyA, eaeA and E-hlyA. It was found that VTEC comprised 6.4% and 21% of the total E. coli isolates from slaughtered small ruminants and slaughterhouses/butcher shops, respectively. In a total of 20 characterized VTEC, the proportion of stx1 and stx2 was 19:1. Through out the study, it was founded that characterized complex VTEC (contain eaeA and/or E-hlyA) possessed 3 virulence patterns including, (VTEC) stx1+eaeA, (VTEC/EHEC) stx1+E-hlyA and (VTEC/EHEC) stx1+eaeA+E-hly A in a percentage of 30%, 25% and 5%, respectively, to the total VTEC isolates. Only one VTEC isolates characterized as E. coli O157, highly pathogenic strain. It was isolated in a percentage of 0.4% to the total E. coli isolates with virulent pattern, stx1, eaeA and E-hlyA. Isolated VTEC showed highly sensitivity to gentamicin and co-trimoxazole and highly resistance ability for tetracycline and ampicillin